Evaluation of resin-based material containing copaiba oleoresin (copaifera reticulata duck): biological effects on the human dental pulp stem cells
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Universidade Federal de Minas Gerais
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Artigo de periódico
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The purpose of this study was to analyze in vitro the biological effects on human dental pulp
stem cells triggered in response to substances leached or dissolved from two experimental cements
for dental pulp capping. The experimental materials, based on extracts from Copaifera reticulata
Ducke (COP), were compared to calcium hydroxide [Ca(OH)2] and mineral trioxide aggregate
(MTA), materials commonly used for direct dental pulp capping in restorative dentistry. For this,
human dental pulp stem cells were exposed to COP associated or not with Ca(OH)2 or MTA. Cell
cytocompatibility, migration, and differentiation (mineralized nodule formation (Alizarin red assay)
and gene expression (RT-qPCR) of OCN, DSPP, and HSP-27 (genes regulated in biomineralization
events)) were evaluated. The results showed that the association of COP reduced the cytotoxicity
of Ca(OH)2. Upregulations of the OCN, DSPP, and HSP-27 genes were observed in response to the
association of COP to MTA, and the DSPP and HSP-27 genes were upregulated in the Ca(OH)2 + COP
group. In up to 24 h, cell migration was significantly enhanced in the MTA + COP and Ca(OH)2 +
COP groups. In conclusion, the combination of COP with the currently used materials for dental pulp
capping [Ca(OH)2 and MTA] improved the cell activities related to pulp repair (i.e., cytocompatibility,
differentiation, mineralization, and migration) including a protective effect against the cytotoxicity of
Ca(OH)2
Abstract
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Resin cements, Fabaceae, Dental pulp capping, Dental pulp, Calcium hydroxide, Biomineralization, Gene expression
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https://www.mdpi.com/2218-273X/10/7/972