Evaluation of resin-based material containing copaiba oleoresin (copaifera reticulata duck): biological effects on the human dental pulp stem cells

Página do item simplificado
dc.creatorRoberta Couto
dc.creatorMaria Fernanda Rodrigues
dc.creatorLeila Ferreira
dc.creatorIvana Márcia Alves Diniz
dc.creatorFernando Silva
dc.creatorTalita Lopez
dc.creatorRafael Rodrigues Lima
dc.creatorMarcia Martins Marques
dc.date.accessioned2022-12-22T00:04:47Z
dc.date.accessioned2025-09-09T00:44:07Z
dc.date.available2022-12-22T00:04:47Z
dc.date.issued2020-06-28
dc.identifier.doihttps://doi.org/10.3390/biom10070972
dc.identifier.issn2218-273X
dc.identifier.urihttps://hdl.handle.net/1843/48335
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBiomolecules
dc.rightsAcesso Aberto
dc.subjectResin cements
dc.subjectFabaceae
dc.subjectDental pulp capping
dc.subjectDental pulp
dc.subjectCalcium hydroxide
dc.subjectBiomineralization
dc.subjectGene expression
dc.titleEvaluation of resin-based material containing copaiba oleoresin (copaifera reticulata duck): biological effects on the human dental pulp stem cells
dc.typeArtigo de periódico
local.citation.epage13
local.citation.issue07
local.citation.spage01
local.citation.volume10
local.description.resumoThe purpose of this study was to analyze in vitro the biological effects on human dental pulp stem cells triggered in response to substances leached or dissolved from two experimental cements for dental pulp capping. The experimental materials, based on extracts from Copaifera reticulata Ducke (COP), were compared to calcium hydroxide [Ca(OH)2] and mineral trioxide aggregate (MTA), materials commonly used for direct dental pulp capping in restorative dentistry. For this, human dental pulp stem cells were exposed to COP associated or not with Ca(OH)2 or MTA. Cell cytocompatibility, migration, and differentiation (mineralized nodule formation (Alizarin red assay) and gene expression (RT-qPCR) of OCN, DSPP, and HSP-27 (genes regulated in biomineralization events)) were evaluated. The results showed that the association of COP reduced the cytotoxicity of Ca(OH)2. Upregulations of the OCN, DSPP, and HSP-27 genes were observed in response to the association of COP to MTA, and the DSPP and HSP-27 genes were upregulated in the Ca(OH)2 + COP group. In up to 24 h, cell migration was significantly enhanced in the MTA + COP and Ca(OH)2 + COP groups. In conclusion, the combination of COP with the currently used materials for dental pulp capping [Ca(OH)2 and MTA] improved the cell activities related to pulp repair (i.e., cytocompatibility, differentiation, mineralization, and migration) including a protective effect against the cytotoxicity of Ca(OH)2
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.initialsUFMG
local.url.externahttps://www.mdpi.com/2218-273X/10/7/972

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Evaluation of resin based material containing copaiba oleoresin copaifera reticulata duck biological effects on the human dental pulp stem cells.pdf
Tamanho:
3.29 MB
Formato:
Adobe Portable Document Format
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG