Anti-serum validation for use in immunohistochemistry for trypanosoma cruzi detection
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Membros da banca
Resumo
The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the
reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft
rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry
(IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. Methods: We
used 1) positive controls (PCs) – 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and
eosin (HE); 2) negative controls – a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic
infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases – 31 EMB of chagasic patients
with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a
hyperimmune serum from T. cruzi-infected rabbits was used. Results: IHC results were positive for 21 of 26 PCs (80.8%) and one
case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological
examination. Conclusions: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may
be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.
Abstract
Assunto
Chagas Disease, Heart Transplantation, Immunohistochemistry, Trypanosoma cruzi
Palavras-chave
Chagas disease, Heart Transplantation, Immunohistochemistry, Trypanosoma cruzi
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Endereço externo
https://doi.org/10.1590/0037-8682-0011-2018