Anti-serum validation for use in immunohistochemistry for trypanosoma cruzi detection

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dc.creatorPaulo Hernane Rabeloazevedo
dc.creatorMarcelo Antônio Pascoal Xavier
dc.creatorGlenda Nicioli da Silva
dc.creatorPriscilla Almeida da Costa Mol
dc.creatorCláudia Martins Carneiro
dc.creatorGeraldo Brasileiro Filho
dc.date.accessioned2024-01-08T18:25:36Z
dc.date.accessioned2025-09-09T00:23:07Z
dc.date.available2024-01-08T18:25:36Z
dc.date.issued2018
dc.format.mimetypepdf
dc.identifier.doi10.1590/0037-8682-0011-2018
dc.identifier.issn16789849
dc.identifier.urihttps://hdl.handle.net/1843/62481
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofRevista da Sociedade Brasileira de Medicina Tropical
dc.rightsAcesso Aberto
dc.subjectChagas Disease
dc.subjectHeart Transplantation
dc.subjectImmunohistochemistry
dc.subjectTrypanosoma cruzi
dc.subject.otherChagas disease
dc.subject.otherHeart Transplantation
dc.subject.otherImmunohistochemistry
dc.subject.otherTrypanosoma cruzi
dc.titleAnti-serum validation for use in immunohistochemistry for trypanosoma cruzi detection
dc.typeArtigo de periódico
local.citation.epage474
local.citation.issue4
local.citation.spage467
local.citation.volume51
local.description.resumoThe detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. Methods: We used 1) positive controls (PCs) – 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls – a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases – 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. Results: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. Conclusions: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE ANATOMIA PATOLÓGICA E MEDICINA LEGAL
local.publisher.initialsUFMG
local.url.externahttps://doi.org/10.1590/0037-8682-0011-2018

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