Msh2 and msh6 genes are methylated in ameloblastoma
Carregando...
Data
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Minas Gerais
Descrição
Tipo
Artigo de periódico
Título alternativo
Primeiro orientador
Membros da banca
Resumo
Objective: To analyze the methylation status of the genes
MSH2, MSH3, and MSH6 in ameloblastomas. Study Design:
Fresh samples of ameloblastoma (group 1, n = 10) and dental fol licles (group 2, n = 10) were digested and their DNA was isolated
for quantitative polymerase chain reaction (qPCR). Methylation
assessment was performed by EpiTect Methyl II PCR Assays
(Qiagen). We also performed immunohistochemical reactions
for MSH2, MSH3, and MSH6 in paraffin sections (group 1). The
results were digitally quantified in all ameloblastomas. Results:
The mean methylation profile of the genes MSH2, MSH3, and
MSH6 in ameloblastoma was not statistically different from den tal follicles. However, a negative correlation for MSH2 (r =
0.7455; P = .0174) and MSH6 (r = 0.8552; P = .0016) protein
expression with the methylation profile was found in ameloblas toma. Conclusion: Although the methylation profile of the genes
in ameloblastoma was not different from dental follicles, our
study shows that epigenetic events are potential regulators of
MSH2 and MSH6 protein expression in ameloblastomas.
Abstract
Assunto
Methylation, Genes, Dental sac, Gene expression, Epigenomics, Ameloblastoma
Palavras-chave
Citação
Departamento
Curso
Endereço externo
https://www.sciencedirect.com/science/article/abs/pii/S2212440320308956