GC-MS/MS versus LC-MS/MS for unambiguous identification of α and β-trenbolone in bovine urine
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Universidade Federal de Minas Gerais
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Artigo de periódico
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This work involves the optimization of LC-MS/MS and GC-MS/MS methods for determination of α and β-trenbolone in bovine urine. The parameters recovery, repeatability and intermediate precision in both methods met the acceptance criteria of the Codex Alimentarius: recovery between 83.5-114% and repeatability and intermediate precision with RSD between 5.1-14.5% and 9.8-25.5% respectively, for concentrations higher than 1 μg kg−1. These methods were applied to the analyses of 5 real samples suspected of contamination. The analyses showed that derivatization with MSTFA/I2 followed by pure MSTFA, was an important tool to prevent false positive results. The use of microwave assisted derivatization reduced significantly the analysis time by GC-MS/MS.
Abstract
Assunto
Urina, Análise, Cromatografia de gás, Espectrometria de massa, Cromatografia líquida, Bovino Saúde
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Bovine urine, Gas chromatography tandem mass spectrometry, Liquid chromatography tandem mass spectrometry, Microwave assisted derivatization, Trenbolone
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https://iicweb.org/scientiachromatographica/v11n4doi10-5935-sc-2019-013en/