Perfil das citocinas IL-4, IL-5, IL-8 e IFN- analisadas por RT-PCRem tecido de mucosa nasal de pacientes portadores de rinite alérgica

Abstract

The allergic rhinitis is an inflammatory reaction of the nasal mucosa, in consequence of the hypersensitive reaction to inhaling allergens and, eventually, alimentary, mediated by IgE, involving different mediators and cytokines cells. The purpose of this study was to evaluate the transcriptions for the following cytokines: IL-4, IL-5, IL- 8 and IFN-ã, particularly important in the allergic nasal process, mainly IL-4 and IL-5,following one of the research lines of the Otorhinolaryngology Service of UFMG. In this study, it was opted to evaluate the atopic patients that were free from allergic crises, with the purpose of knowing the cytokines expressions in this period. Another prospective and transversal study was carried out, selecting 30 patients, 13 of these pacients were paucisymptomatic and 17 non atopic pacients. The groups wereselected throughout the history, from the otorhinolaryngologic clinical exam and from the skin allergic test Prick test. The cytokines profile was researched in fragments of the nasal mucosa, using the reverse transcription semi quantitative polymerase chain reaction (RT-PCR), wich has been chosen by the reason that it presented a good reproductivity and specificity, using beta-actin as its control. It was observed theincrease of IL-4 with statistic significance, one strong trend to the IL-5 rise, while the values for IL-8, and IFN-ã cytokines remained homogenous compared to the non atopic group control.