Edaravone inhibits the production of reactive oxygen species in phagocytosis- and pkc-stimulated granulocytes from multiple sclerosis patients edaravone modulate oxidative stress in multiple sclerosis
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Universidade Federal de Minas Gerais
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BACKGROUND: Oxidative stress is associated with the pathogenesis of MS. Edaravone (EDV) has been proposed as a therapeutic resource for central nervous system diseases, and it was effective in reducing oxidative stress. However, the antioxidant mechanisms of EDV are poorly studied.
OBJECTIVE: This study aimed to evaluate the effects of EDV on resting, phagocytosis, and PKC-activated granulocytes derived from MS patients and a healthy control group.
METHODS: The effects of EDV on ROS production in phagocytosis (ROS production in the presence of opsonized particles) and PKC-stimulated granulocytes were evaluated in a luminol-dependent chemiluminescence method. Calphostin C was used in some experiments to compare with those of EDV.
RESULTS: EDV inhibited ROS production in phagocytosis of opsonized particles and PKC-stimulated granulocytes from MS patients and healthy control group. In the presence of calphostin C, the inhibition of ROS production was similar to that observed with EDV.
CONCLUSION: These findings suggest the involvement of EDV on the ROS-PKC-NOX signaling pathways modulating oxidative stress in MS. EDV represents a promising treatment option to control oxidative innate immune response for MS.
KEYWORDS: edaravone, multiple sclerosis, innate immunity, reactive oxygen species, phagocytosis, protein kinase C.
Abstract
Assunto
Edaravone, Esclerose Múltipla, Estresse Oxidativo
Palavras-chave
Multiple Sclerosis, Edaravone, Oxidative stress modulation
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https://journals.sagepub.com/doi/10.1177/11795735221092524