Nestin and neuron-glial antigen 2 transgenes unveil progenitor units in murine salivary glands
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Objective: Undifferentiated cells play pivotal roles in sustaining tissue homeostasis during physiological turnovers and after tissue impairment. Nestin and Neuron-glial antigen 2 (NG2) are markers frequently deployed to distinguish progenitor populations. In the salivary gland scenario, these markers remain largely unknown. Particularly for a double-labeled group of progenitor cells (NG2+Nestin+), their phenotype and distribution have never been explored in freshly isolated tissues. Herein, we analyzed a subset of plastic cells that express Nestin and NG2 near the ducts and in the periacinar region of the major salivary glands of murine samples.
Design: The major salivary glands tissues of Nestin-GFP/NG2-DsRed mice were analyzed under a fluorescence microscope. The cells marked by GFP and DsRed were counted in the merged image component of random representative images obtained for each gland sample at × 20 magnification.
Results: In the parotid, submandibular, and sublingual glands, the population of cells exclusively expressing Nestin was more abundant. There was a predominance of Nestin, NG2, and double-labeled cells in the submandibular gland compared to the parotid gland, mainly near the ductal system. Of note, the sublingual and parotid glands had similar populations of Nestin+ and NG2+, especially in acini, and some positive cells were observed surrounding ducts.
Abstract
Assunto
Nestin, Salivary glands, Parotid gland, Stem cells, Sublingual gland, Submandibular gland., Submandibular gland, Homeostasis
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Nestin, Neuron-glial antigen 2, Salivary gland, Parotid gland, Stem cell, Sublingual gland, Submandibular gland
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https://www.sciencedirect.com/science/article/pii/S0003996921003071?via%3Dihub