An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
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Universidade Federal de Minas Gerais
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Artigo de periódico
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An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
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Resumo
Prednisone is an anti-inflammatory steroid drug widely used in clinical practice. However, no highperformance liquid chromatographic (HPLC) method has been described in the literature for the
determination of prednisone in capsules until now. Thus, an HPLC method was developed using a
C18 (250x4.0, 5 µm) column, with methanol:water (70:30) as mobile phase at a flow rate of 1 mL/min
and detection at 240 nm. The developed method was validated following current Brazilian legislation.
Additionally, linearity was assessed by evaluating the assumptions of normality, homoscedasticity, and
independency of residuals, and the fit to the linear model. The method showed linearity (r2
>0.99) over
the range of 14.0-26.0 µg/mL, selectivity, precision (RSD<2.0%), robustness, and accuracy (average
recovery of 100.05%). The chromatographic procedure was applied for assay and uniformity content
determination of three different batches of prednisone capsules, showing to be suitable for their quality
control
Abstract
Assunto
Química, Prednisona, Cápsulas (Farmácia)
Palavras-chave
Prednisone, Capsules, High performance liquid chromatography/validation, Linearity assessment
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https://www.scielo.br/j/bjps/a/9WrhFmD4HpVPHYKqWwJKGpq/?lang=en