Análise dos marcadores de dinâmica mitocondrial no carcinoma adenoide cístico

Abstract

Mitochondrial fission and fusion processes are known as mitochondrial dynamics and the occurrence of imbalances in the mitochondrial activity is related to the pathogenesis of many human cancers. However, the importance of mitochondrial dynamics in malignant salivary gland tumours remains unknown. Therefore, we aimed to investigate its prognostic significance in adenoid cystic carcinoma (AdCC). Fifty-seven formalin-fixed paraffin-embedded cases were retrieved and disposed in tissue microarray. Histological sections were submitted to immunohistochemical reactions against AMT, DRP1, FIS1, MFN1, MFN2 and OPA1 proteins. Clinical data were retrieved from the patients’ medical files, including specific and disease-free survival data. It was observed that 50.9% of the cases were strongly positive for AMT and DRP1, and 49.1%, 21.1%, 22.8% and 24.6% strongly positive for FIS1, MFN1, MFN2 and OPA1, respectively. Reactions were observed in both epithelial and myoepithelial components of the tumour. The higher expression of MFN2 was associated with solid microscopic pattern (p = .016). DRP1 overexpression showed a trend towards a shorter survival (p = .054), while negative/weak OPA1 showed a trend towards a lower disease free survival (DFS) (p=.051) in the univariate analysis, but no mitochondrial marker represented an independent prognostic determinant under multivariate analysis. In conclusion, mitochondrial dynamics markers do not seem to carry a prognostic significance for AdCC patients, but these proteins may play an important role in its pathogenesis.