Estudo comparativo de mastócitos e células de langerhans na doençaperiodontal em indivíduos HIV-positivos usuários de HAART e HIV-negativos

Abstract

The goal of this seccional study was to analyze and compare the c-kit+ and tryptase+ mast cells densities, frequency and Langerhans S100+ cells densities by immunochemistry techniques in plaque induced human gingivitis and periodontitis samples, HIV-positive andHIV-negative subjects. Additionally, to evaluate correlation among densities and frequency of these cells with CD4+, CD8+ T cells and viral load levels in HIV-positive subjects. 50 biopsies samples were obtained and presenting in four groups: 1) 15 subjects with moderate chronic periodontitis (MCP) HIV-positive, 2) 15 subjects with moderate chronicperiodontitis (MCP) HIV-negative, 3) 10 subjects with gingivitis (G) HIV-positive, 4) 10 subjects HIV-negative and G. All HIV-positive subjects were taking HAART (Highly Active Antiretroviral Therapies). No statistically significant difference of density and frequency among ckit+ and tryptase+ mast cells were observed and the groups. In the gingivitis andperiodontitis HIV groups were observed a decrease of frequency of tryptase+ mast cells with infiltrate inflammatory increase. In case of ckit there was a decrease of frequency only in gingivitis HIV-negative group. Total Langerhans cells were observed higher MCP HIVpositivethan the number of HIV-negative group. No statistically significant difference was observed when analyzed by localization tissue even with infiltrate inflammatory increase. No correlation statistically significant was observed among S100+, ckit+ and tryptase+cells and blood levels of CD4, CD8 and viral load. The HAART therapy can be theresponsible to maintain an efficient immune response in HIV-positive subjects. In this way, propitiate density and frequency of mast cells just like HIV-negative subjects with gingivitis and periodontitis. Additionally, the HAART therapy is a CL protector factor, because is responsible to decrease viral load and than cause lower aggression to this cells in thetissue with gingivitis and periodontitis.