Cytotoxicity, biocompatibility, and biomineralization of the new high-plasticity mta material
Carregando...
Data
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Minas Gerais
Descrição
Tipo
Artigo de periódico
Título alternativo
Primeiro orientador
Membros da banca
Resumo
Introduction: Mineral trioxide aggregate (MTA) has
excellent biological properties, but its handling proper-
ties have been criticized for both ProRoot MTA (Tulsa
Dental Products, Tulsa, OK) and white MTA-Angelus
(MTA-Ang; Angelus Ind ustria de Produtos Odon-
tol ogicos S/A, Londrina, PR, Brazil). Angelus MTA HP
(high plasticity) (Angelus Ind ustria de Produtos Odon-
tol ogicos S/A) has been introduced recently. Considering
the importance of biological properties of materials that
will be in contact with the tissues, this study evaluated
the cytotoxicity, biocompatibility, and biomineralization
of MTA HP compared with white MTA-Ang. Methods:
L929 fibroblast cell lines were cultured, and cell viability
was assessed at 6, 24, 48, and 72 hours using the ala-
mar Blue assay (Thermo Fisher Scientific, Waltham,
MA). A subcutaneous implant test was performed with
polyethylene tubes containing 1 of the materials or
empty tubes (control) using 20 Wistar rats. After 7 and
30 days of implantation, the tubes with surrounding tis-
sues were removed for analysis using hematoxylin-eosin
or von Kossa stain or they remained unstained for obser-
vation under polarized light. The results were statisti-
cally analyzed (P < .05). Results: A significant
increase in cell viability for MTA HP was observed after
24, 48, and 72 hours compared with the control
(P < .05). At 72 hours, MTA HP exhibited a higher
viability compared with white MTA-Ang (P < .05). His-
tologic analysis performed at 7 days showed moderate
inflammation and a thick fibrous capsule in all groups
(P > .05). At 30 days, mild inflammation and a thin
fibrous capsule were observed in all groups (P > .05).
All materials had structures positive for von Kossa and
birefringent to polarized light. Conclusions: MTA HP
showed biocompatibility and biomineralization similar
to MTA-Ang. In addition, MTA HP showed increased
fibroblast cell viability compared with white MTA-Ang
after a longer period
Abstract
Assunto
Materials testing, Biomineralization
Palavras-chave
Biocompatibility, Biomineralization ability, Cytotoxicity, Mineral trioxide aggregate
Citação
Departamento
Curso
Endereço externo
https://www.sciencedirect.com/science/article/pii/S0099239916310640?via%3Dihub