Avaliação de técnicas de biópsia hepática e renal em ovinos

Abstract

Since there are no studies that compare hepatic biopsy techniques and few on renal biopsies inovines, a study was developed comparing eight hepatic biopsy techniques and three renal biopsytechniques in this species. Eight crossbred Santa Inês ewe lambs (17.95kg ± 2.71) and nine ewes(26.64kg ± 4.86) were subjected to serial hepatic and renal biopsies, respectively. Amongst thehepatic procedures, six biopsy techniques were performed using two needles, Menghinimodified (M) and tru-cut semi-automatic (T), using the blind percutaneous technique,ultrasound-guided (US) and monitored by videolaparoscopy (VL). In the other two procedures,the use of laparoscopic biopsy forceps and the hepatic tissue resection technique by VL wasevaluated. Renal biopsy specimens were obtained by the keyhole technique, ultrasound-guided,using T needles and VL with laparoscopic forceps. Adverse hematological or biochemical signswere not observed in the animals after the hepatic or renal biopsies and the clinical signs wereslight and transitory, except for a case of urethral obstruction caused by the formation of bloodclots. In general, the use of US and VL in the percutaneous biopsies did not result in asignificant increase of weight or histological quality of the hepatic specimens. The use of Mneedles showed a greater difficulty at tissue sampling, higher weight oscillations, and a lowerquality of the specimens obtained than when using T needles. Specimen quality obtained byboth VL techniques was similar; however, the use of the resection technique resulted in highersample weight and the formation of hepatic adhesions. The keyhole technique was associatedwith greater and more severe evidence of hematuria, damage to the renal tissue and a single caseof obstruction of the urinary flow. The VL technique was associated with discrete evidence ofhematuria and medium to superior weight of the specimens