Dna methylation patterns of immune response-related genes in inflammatory external root resorption
Carregando...
Data
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Minas Gerais
Descrição
Tipo
Artigo de periódico
Título alternativo
Primeiro orientador
Membros da banca
Resumo
Abstract: Inflammatory external root resorption (IERR) is a pathological
process defined by the progressive loss of dental hard tissue, dentin, and
cementum, resulting from the combination of the loss of external root
protective apparatus and root canal infection. It has been suggested that
healing patterns after tooth replantation may be influenced by the genetic
and immunological profiles of the patients. The purpose of the present
investigation was to evaluate the DNA methylation patterns of 22 immune
response-related genes in extracted human teeth presenting with IERR.
Methylation assays were performed on samples of root fragments
showing IERR and compared with healthy bone tissue collected during
the surgical extraction of impacted teeth. The methylation patterns were
quantified using EpiTect Methyl II Signature Human Cytokine Production
PCR Array. The results revealed significantly higher hypermethylation of
the FOXP3 gene promoter in IERR (65.95%) than in the bone group (23.43%)
(p < 0.001). The ELANE gene was also highly methylated in the pooled
IERR sample, although the difference was not statistically significant
(p= 0.054). Our study suggests that the differential methylation patterns
of immune response-related genes, such as FOXP3 and ELANE, may be
involved in IERR modulation, and this could be related to the presence of
root canal infection. However, further studies are needed to corroborate
these findings to determine the functional relevance of these alterations
and their role in the pathogenesis of IERR.
Abstract
Assunto
Epigenomics, Methylation, Inflammation, Replantation, Root resorption, Tooth extraction
Palavras-chave
Epigenomics, Methylation, Inflammation, Replantation, Root resorption
Citação
Departamento
Curso
Endereço externo
https://www.scielo.br/j/bor/a/34YhKTPhSWG3HVmZPKBMfdv/?lang=en