Perfil de expressão gênica de il-5, gm-csf, tgf-1, eotaxina na poliposenasossinusal eosinofílica pós-cirurgia endoscópica e efeito da mitomicina c tópica

Abstract

Nasosinusal polyposis is a degeneration of the mucosa of the nose and paranasal sinuses with the participation of several inflammatory cytokines. Objective: To compare the differences in the expression of inflammatory genes in eosinophilic nasosinusal polyposis prior and after the topical application of Mitomycin C in the maxillary sinus, and to relate it to their expression in normal tissue. Casuistic and Method: twenty patients with nasosinusal polyposis and six patients with pituitary adenoma were selected and underwent endoscopic surgical treatment. Maxillary sinus mucosa was biopsied at surgery and the left maxillary sinus was submitted to topical application of 3 ml of Mitomycin C O.5 mg/ml for 5 minutes. In the 21st day after surgery a new biopsy was collected from the maxillary sinuses. In the six patients who underwent transsphenoidal surgery for removal of pituitary tumors a biopsy of the normal mucosa of the sphenoid sinus was carried out. The samples were analyzed through RT-qPCR for the expression of IL5, eotaxin, granulocyte macrophage colony stimulating factor and that also transforming growth factor beta-1. Results: It was observed, in the diseased mucosa, an asymmetric distribution for the values of the cytokines. The values of eotaxin diminished after treatment. In the left maxillary sinus, the expression of IL5 was greater in the trans-operatory period (prior to the application of Mitomycin C) compared to normal tissue and to the 21st day after the application of the medication. Conclusion: There was indication of the action of Mitomycin C in the subexpression of IL5. It was also observed the recommendation of the benefit of surgery and corticoid for eotaxin. The expression of cytokines had asymmetrical values, and increased in relation to normal tissue except for beta-1 growth factor.