Aplicações de espalhamento Raman estimulado em biomedicina

Abstract

In this dissertation, the techniques of Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) are detailed, highlighting their roles as advanced optical methods for investigating biological materials with high resolution and sensitivity. These techniques allow for the acquisition of detailed information about the composition and structure of biological samples, enabling non-invasive and real-time analysis. In addition to reviewing the applications of CARS and SRS techniques in the literature, this study utilizes CARS and SRS to characterize and analyze different biological materials. The study focuses on leishmania, optic nerves of mice under different degeneration conditions, including Wallerian degeneration (WD) and the Huntington’s disease (HD) model, and trigeminal nerves of mice, where it was possible to differentiate between two distinct models. In all cases, the SRS and CARS techniques proved effective in the characterization and analysis of the studied biological materials.