L-carnitine added to post-thawed semen acts as an antioxidant and a stimulator of equine sperm metabolism
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Membros da banca
Resumo
The objective of this study was to enhance the in vitro sperm quality and in vivo fertil-ity of frozen-thawed equine semen by the addition of l-carnitine (LC) to post-thawedsemen. Different concentrations of LC were added to thawed samples to obtain fourtreatments control and 0.5, 1 and 2 mM LC. In the in vitro experiments, sperm motil-ity and kinematics, membrane integrity and intracellular calcium ion concentration([Ca 2+] i ) were investigated, and the antioxidant bioactivity of LC was assessed bymeasuring hydrogen peroxide and nitrite concentrations (NO2−). The fertility rate wasassessed via the artificial insemination of mares. The treatment with 1 mM LC in-creased sperm [Ca 2+] i (60.6 ± 0.05 AU), reduced nitrite concentration (39.1 ± 14.9 µM/µg protein), increased the sperm straightness percentage (STR: 78.3 ± 5.3%) and in-creased the pregnancy rate (75%) as compared to the control ([Ca 2+] i 48.4 ± 0.05 AU,NO2− concentration 63.1 ± 14.4 µM/µg protein, STR 67.5 ± 7.9%, 12.5% pregnancyrate, p < 0.05). These results suggest that 1 mM LC acts as an antioxidant and stimula-tor of sperm metabolism in post-thawed equine semen, increasing the fertility rate.Thus, addition of LC might be an alternative to improve the fertility of poor qualitypost-thawed equine semen.
Abstract
Assunto
Equino, Sêmen congelado
Palavras-chave
Stallion, Reproduction, Cryopreservation, Frozen semen, Fertility
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https://onlinelibrary.wiley.com/doi/10.1111/and.14338