L-carnitine added to post-thawed semen acts as an antioxidant and a stimulator of equine sperm metabolism

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dc.creatorMonique de Albuquerque Lagares
dc.creatorAdalgiza Souza Carneiro de Rezende
dc.creatorMarina Morra Freitas
dc.creatorRubens Stahlberg
dc.creatorRafael Romero Nicolino
dc.creatorGrazielle Caroline da Silva
dc.creatorSteyner Franca Cortes
dc.creatorFabio Henrique Marques Moreira
dc.creatorFranciele Caroline Dias Neves
dc.creatorNatália de Castro Alves
dc.creatorRodrigo Novaes Viegas
dc.creatorThiago Frederico Diniz
dc.creatorVirginia Soares Lemos
dc.date.accessioned2025-05-28T12:33:27Z
dc.date.accessioned2025-09-09T00:41:59Z
dc.date.available2025-05-28T12:33:27Z
dc.date.issued2022
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1111/and.14338
dc.identifier.issn03034569
dc.identifier.urihttps://hdl.handle.net/1843/82551
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofAndrologia
dc.rightsAcesso Restrito
dc.subjectEquino
dc.subjectSêmen congelado
dc.subject.otherStallion
dc.subject.otherReproduction
dc.subject.otherCryopreservation
dc.subject.otherFrozen semen
dc.subject.otherFertility
dc.titleL-carnitine added to post-thawed semen acts as an antioxidant and a stimulator of equine sperm metabolism
dc.typeArtigo de periódico
local.citation.issue3
local.citation.spagee14338
local.citation.volume54
local.description.resumoThe objective of this study was to enhance the in vitro sperm quality and in vivo fertil-ity of frozen-thawed equine semen by the addition of l-carnitine (LC) to post-thawedsemen. Different concentrations of LC were added to thawed samples to obtain fourtreatments control and 0.5, 1 and 2 mM LC. In the in vitro experiments, sperm motil-ity and kinematics, membrane integrity and intracellular calcium ion concentration([Ca 2+] i ) were investigated, and the antioxidant bioactivity of LC was assessed bymeasuring hydrogen peroxide and nitrite concentrations (NO2−). The fertility rate wasassessed via the artificial insemination of mares. The treatment with 1 mM LC in-creased sperm [Ca 2+] i (60.6 ± 0.05 AU), reduced nitrite concentration (39.1 ± 14.9 µM/µg protein), increased the sperm straightness percentage (STR: 78.3 ± 5.3%) and in-creased the pregnancy rate (75%) as compared to the control ([Ca 2+] i 48.4 ± 0.05 AU,NO2− concentration 63.1 ± 14.4 µM/µg protein, STR 67.5 ± 7.9%, 12.5% pregnancyrate, p < 0.05). These results suggest that 1 mM LC acts as an antioxidant and stimula-tor of sperm metabolism in post-thawed equine semen, increasing the fertility rate.Thus, addition of LC might be an alternative to improve the fertility of poor qualitypost-thawed equine semen.
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE FARMACOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA
local.publisher.departmentVET - DEPARTAMENTO DE ZOOTECNIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/and.14338

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