Comparison between enzyme immunoassays performed on samples of dried blood and serum for toxoplasmosis prenatal screening: population-based study
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Membros da banca
Resumo
Objective Most prenatal screening programs for toxoplasmosis use immunoassays in
serum samples of pregnant women. Few studies assess the accuracy of screening tests
in dried blood spots, which are of easy collection, storage, and transportation. The
goals of the present study are to determine the performance and evaluate the
agreement between an immunoassay of dried blood spots and a reference test in
the serum of pregnant women from a population-based prenatal screening program
for toxoplasmosis in Brazil.
Methods A cross-sectional study was performed to compare the immunoassays
Imunoscreen Toxoplasmose IgM and Imunoscreen Toxoplasmose IgG (Mbiolog Diagnósticos,
Ltda., Contagem, Minas Gerais, Brazil)in dried blood spots with the enzymelinked
fluorescent assay (ELFA, BioMérieux S.A., Lyon, France) reference standard in the
serum of pregnant women from Minas Gerais Congenital Toxoplasmosis Control
Program.
Results The dried blood spot test was able to discriminate positive and negative
results of pregnant women when comparedwith the reference test, with an accuracy of
98.2% for immunoglobulin G (IgG), and of 95.8% for immunoglobulin M (IgM).
Conclusion Dried blood samples are easy to collect, store, and transport, and they
have a good performance,making this a promisingmethod for prenatal toxoplasmosis
screening programs in countries with continental dimensions, limited resources, and a
high prevalence of toxoplasmosis, as is the case of Brazil.
Abstract
Assunto
Medicina
Palavras-chave
Prenatal, Congenital
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Endereço externo
https://lp.thieme.de/journals/rbgo-gynecology-und-obstetrics/0100-7203