Utilização de água de coco (cocos nucifera) industrializada como conservante para Trypanosoma vivax

Abstract

Trypanosomiasis in ruminants is a disease of great importance in South America. A major difficulty for parasitological diagnosis is the parasite viability after the blood is collected to perform the analysis. The Woo test is the widely used test when the parasitemia of the sample is low, because it has greater sensitivity. Coconut water has been used for the cultivation of plant cells, adjuvant for culture of fungi and protozoa, and as diluent semen from several species. This study aimed to use the coconut water industrialized as adjuvant in the diagnosis of Trypanosoma vivax by the method of Woo. The study was conducted in July 2013, based on the analysis of three different parasitemia, 550000, 100000 and 1000 parasites/mL. Each parasitemia had 60 samples, including 30 samples in the control group G1 (1mL infected blood ) and the other 30 samples in the treated group G2 (1 mL of infected blood plus 1 ml of coconut water processed). All samples were analyzed in five times (T1: time zero, T2: 6 hours after dilution, T3: 12 hours after dilution, T4 for 24 hours after dilution, and T5: 48 hours after dilution). For statistical analysis was used the chi- square and McNemar tests, with minimun 95 % significance (p < 0.05), in SAS program. The results obtained showed significant differences between the treatments. The use of coconut water increased the viability of hemoparasite at times T2, T3 and T4. The three parasitaemia, when analyzed separately, showed different results and pointed their influence, as well as the time involved in the study.