The role of 5-lipoxygenase in Aggregatibacter actinomycetemcomitans-induced alveolar bone loss
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Resumo
Aim: Leukotrienes (LTs) are pro-inflammatory lipid mediators formed by the enzyme 5-lipoxygenase (5-LO). The involvement of 5-LO metabolites in periodontal disease (PD) is not well defined. This study aimed to assess the role of 5-LO in experimental PD induced by Aggregatibacter actinomycetemcomitans (Aa).
Material and methods: In vivo experiments were carried out using SV129 wild-type (WT) and 5-LO-deficient (5lo-/- ) mice inoculated with Aa. Osteoclasts were stimulated in vitro with AaLPS in the presence or not of selective inhibitors of the 5-LO pathway, or LTB4 or platelet-activating factor (PAF), as PAF has already been shown to increase osteoclast activity.
Results: In 5lo-/- mice, there were no loss of alveolar bone and less TRAP-positive osteoclasts in periodontal tissues, after Aa inoculation, despite local production of TNF-α and IL-6. The differentiation and activity of osteoclasts stimulated with AaLPS were diminished in the presence of BLT1 antagonist or 5-LO inhibitor, but not in the presence of cysteinyl leukotriene receptor antagonist. The osteoclast differentiation induced by PAF was impaired by the BLT1 antagonism.
Conclusion: In conclusion, LTB4 but not CysLTs is important for Aa-induced alveolar bone loss. Overall, LTB4 affects osteoclast differentiation and activity and is a key intermediate of PAF-induced osteoclastogenesis.
Abstract
Assunto
Aggregatibacter actinomycetemcomitans, Alveolar bone loss, Leukotrienes, Periodontal diseases, Enzymes, Proteins, Osteoclasts, Leukotriene B4, Osteogenesis, Platelet activating factor
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Aggregatibacter actinomycetemcomitans, 5-lipoxygenase, Alveolar bone loss, Leukotriene, Periodontal disease
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https://onlinelibrary.wiley.com/doi/10.1111/jcpe.12755