The role of 5-lipoxygenase in Aggregatibacter actinomycetemcomitans-induced alveolar bone loss

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dc.creatorMila Fernandes Moreira Madeira
dc.creatorDanielle Glória Souza
dc.creatorCelso Martins Queiroz-junior
dc.creatorJôice Dias Corrêa
dc.creatorSílvia Maria Cordeiro Werneck
dc.creatorFabiana Simão Machado
dc.creatorThiago Mattar Cunha
dc.creatorGustavo Pompermaier Garlet
dc.creatorMauro Martins Teixeira
dc.creatorTarcília Aparecida da Silva
dc.date.accessioned2025-07-02T14:28:17Z
dc.date.accessioned2025-09-09T00:17:32Z
dc.date.available2025-07-02T14:28:17Z
dc.date.issued2017-08
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/jcpe.12755
dc.identifier.issn1600-051X
dc.identifier.urihttps://hdl.handle.net/1843/83279
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Clinical Periodontology
dc.rightsAcesso Restrito
dc.subjectAggregatibacter actinomycetemcomitans
dc.subjectAlveolar bone loss
dc.subjectLeukotrienes
dc.subjectPeriodontal diseases
dc.subjectEnzymes
dc.subjectProteins
dc.subjectOsteoclasts
dc.subjectLeukotriene B4
dc.subjectOsteogenesis
dc.subjectPlatelet activating factor
dc.subject.otherAggregatibacter actinomycetemcomitans
dc.subject.other5-lipoxygenase
dc.subject.otherAlveolar bone loss
dc.subject.otherLeukotriene
dc.subject.otherPeriodontal disease
dc.titleThe role of 5-lipoxygenase in Aggregatibacter actinomycetemcomitans-induced alveolar bone loss
dc.typeArtigo de periódico
local.citation.epage802
local.citation.issue8
local.citation.spage793
local.citation.volume44
local.description.resumoAim: Leukotrienes (LTs) are pro-inflammatory lipid mediators formed by the enzyme 5-lipoxygenase (5-LO). The involvement of 5-LO metabolites in periodontal disease (PD) is not well defined. This study aimed to assess the role of 5-LO in experimental PD induced by Aggregatibacter actinomycetemcomitans (Aa). Material and methods: In vivo experiments were carried out using SV129 wild-type (WT) and 5-LO-deficient (5lo-/- ) mice inoculated with Aa. Osteoclasts were stimulated in vitro with AaLPS in the presence or not of selective inhibitors of the 5-LO pathway, or LTB4 or platelet-activating factor (PAF), as PAF has already been shown to increase osteoclast activity. Results: In 5lo-/- mice, there were no loss of alveolar bone and less TRAP-positive osteoclasts in periodontal tissues, after Aa inoculation, despite local production of TNF-α and IL-6. The differentiation and activity of osteoclasts stimulated with AaLPS were diminished in the presence of BLT1 antagonist or 5-LO inhibitor, but not in the presence of cysteinyl leukotriene receptor antagonist. The osteoclast differentiation induced by PAF was impaired by the BLT1 antagonism. Conclusion: In conclusion, LTB4 but not CysLTs is important for Aa-induced alveolar bone loss. Overall, LTB4 affects osteoclast differentiation and activity and is a key intermediate of PAF-induced osteoclastogenesis.
local.identifier.orcidhttps://orcid.org/0000-0002-1770-9978
local.identifier.orcidhttps://orcid.org/0000-0002-7884-7709
local.identifier.orcidhttps://orcid.org/0000-0003-0170-6163
local.identifier.orcidhttps://orcid.org/0000-0001-9272-5209
local.identifier.orcidhttps://orcid.org/0000-0003-1084-0065
local.identifier.orcidhttps://orcid.org/0000-0002-5071-8382
local.identifier.orcidhttps://orcid.org/0000-0002-6944-3008
local.identifier.orcidhttps://orcid.org/0000-0001-9623-7835
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE FARMACOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jcpe.12755

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