Validação de uma PCR em tempo real para o diagnóstico da doença de Aujeszky

Abstract

Aujeszky's disease (AD) is an contagious infectious disease of viral etiology that constitutes a major obstacle to the exploration and international trade of swine. The Suid Herpesvirus 1 (SuHV-1) belongs to the subfamily Alphaherpesvirinae and among other features has the ability to establish latent infections in sensory neurons. Considered as a notifiable disease by the OIE, is present in the list of diseases of importance economic and epidemiology in swine. The production systems become more intensive and confined, and consequently the risk of spreading disease also increased. The adoption of strategies and public programs for control and eradication of AD, in order to prevent the spread of the disease, it is necessary to prevent extensive damage to the pig farm in Brazil. The aim of this work was to validate a real time PCR for the diagnosis of AD and to verify the performance of different systems: SybrGreen I, hybridization probe and Plexor. To check the performance of the following criteria were used: specificity and selectivity, sensitivity, limit of detection, repeatability and reproducibility and robustness. The best performance was achieved using the primers and probes designed to target gB, this fact influenced the decision-making in the choice of methodology to be validated and available in the Laboratory of Molecular Biology of the LANAGRO-MG, as a tool for the AD diagnostic in Brazil.