Evaluation of the cytotoxicity and biocompatibility of new resin epoxy-based endodontic sealer containing calcium hydroxide
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Membros da banca
Resumo
Introduction: Many endodontic sealers are available,
but the search for the ideal sealer continues. This study
evaluated the cytotoxicity and biocompatibility of Sealer
Plus, a new resin epoxy–based endodontic sealer con-
taining calcium hydroxide. AH Plus, Endofill, and Simpli-
Seal endodontics sealers were used for comparison.
Methods: L929 fibroblasts were cultured, and an MTT
assay was used to determine the cytotoxicity of the
sealer extracts at 6, 24, 48, and 72 hours. Tubes contain-
ing materials or empty tubes for control were inserted
into the subcutaneous tissues of 20 rats. After 7 and
30 days, the rats were killed, and the tubes were
removed with the surrounding tissues for histologic
analysis. The data were submitted to statistical tests
(P < .05). Results: Undiluted Sealer Plus exhibited
less cytotoxicity compared with other undiluted extracts
at 6 hours (P < .05), and cell viability was higher for all
Sealer Plus extracts after 24 hours (P < .05). At 48 hours,
the undiluted and ½ Sealer Plus dilution were the ex-
tracts with less cytotoxicity (P < .05). At 72 hours, cell
viability was higher for the undiluted and ½ Sealer
Plus dilution compared with the other sealers
(P < .05). At 7 days, Endofill and SimpliSeal had higher
inflammation compared with the control and Sealer Plus
(P < .05); AH Plus had moderate inflammation (P > .05).
At 30 days, control, Sealer Plus, and AH Plus had less
inflammation (P < .05). The fibrous capsule was thick
at 7 days and thin at 30 days, except for SimpliSeal.
Conclusions: In general, Sealer Plus promoted greater
cell viability and was more biocompatible compared
with the other sealers.
Abstract
Assunto
Materials testing, Calcium hydroxide, Epoxy resins, Noxae
Palavras-chave
Biocompatibility, Calcium hydroxide, Cytotoxicity, Endodontic sealers, Resin epoxy
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https://www.sciencedirect.com/science/article/pii/S0099239917309056?via%3Dihub