Cinética da proliferação e migração de fibroblastos isolados a partir da pele de couro cabeludo de neonatos e lactentes com hidrocefalia

Abstract

Introduction: Cerebrospinal fluid wound leakage and suture dehiscence are complications that can occur in the postoperative period of cerebrospinal fluid shunts from hydrocephalic infants. In the literature, it is already known a higher prevalence of these complications below one year of life and that cicatrization differs acording to age. Objetives: To evaluete the rate of proliferation and migration of fibroblast in neonates, compared to infants, operated on hydrocephalus, also observing nutritional status. Material and Method: Skin biopsies from the scalp of newborns and infants were performed during hydrocephalus surgery of different etiologies. The skins were treated and cultured. In the fourth passage tests were carried out. The cell growth curve was constructed, the cell viability that was measured by the metabolism of MTT (3- (4,5 dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) at pre-established times and the rate of fibroblast migration by measuring fills of scratch on the culture plate, calculated from photographs. The triplicates were: 3 nourished newborns operated at 1 month of age, 3 malnourished newborns operated at 1 month of age, 3 nourished infants operated between 1 and 6 months of life, and 3 malnourished infants operated between 1 and 6 months of life. Results: The cellular growth of the groups operated before one month of life behaved significantly differently when compared to those operated after the first month of life. The fibroblasts of children operated before one month of life had a sigmoid growth profile and those operated from one month an exponential profile. Nutritional status influenced cell growth. There was no significant difference in the metabolism of MTT, that is, cell viability, considering age and nutrition. About the migration ability, shows that children fibroblast of the same age group behaves in a similar way, but when compared to the surgical moment, again the group operated before one month of life migrates significantly slower. Conclusion: Neonatal fibroblasts have an initial doubling capacity similar to infants, but they lose this ability more rapidly. Malnutrition affects the growth rate in the first month of life. The neonatal fibroblast is slower in the first 24 hours, considering the migration. The metabolic pathways responsible for these results should be studied, and we advise that if possible children should be operated on after the first month of life or operative points should be maintained longer than usual when operated in the first month of life.