Loss of heterozygosity and cell proliferation in oral lichenoid lesions related to amalgam
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Membros da banca
Resumo
Oral lichenoid lesions related to amalgam (OLLRA) and oral lichen planus (OLP) share clinical and histopathologic features with molecular pathogenesis under clarification.
Objective
Here investigated loss of heterozygosity (LOH) in OLLRA, OLP and normal oral mucosa (NOM) using polymorphic microsatellite markers close to tumor suppressor genes and evaluated the index of cell proliferation in paraffin-embedded tissues.
Study Design
The sample comprised 10 OLLRAs, 10 OLPs and 8 NOMs matched by gender and age of the patient. LOH was assessed on 9p (D9S157, D9S162, D9S171), 11q (D11S1369) and 17p (TP53, AFM238WF2) chromosomes loci. The cell proliferation was assessed by immunohistochemical expression of Ki-67 (MIB-1).
Results
LOH was identified in 5 OLLRAs and in 2 OLPs in at least 1 marker while NOM showed no LOH. The index of Ki-67 expression in OLLRA was considered low (below 25%). There was no association between cell proliferation and LOH.
Conclusions
This study demonstrates that OLLRA and OLP have LOH in the 9p and 17p chromosomal regions and low expression of ki-67. Despite the lack of association between LOH and cell proliferation, it can be assumed that LOH occurs in OLLRA and it should be considered in the pathogenesis of this lesion.
Abstract
Assunto
Evaluation study, Cell proliferation, Genes, Loss of heterozygosity, Lichen planus, oral, Wounds and injuries, Dental amalgam, Mouth mucosa, Chromosomes, human, pair 9, Chromosomes, human, pair 11, Chromosomes, human, pair 17, Ki-67 antigen, Immunohistochemistry
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Endereço externo
https://www.oooojournal.net/article/S2212-4403(17)30720-4/fulltext