Aspectos histológicos, parasitológicos e imunológicos da infecção experimental por Leishmania (Leishmania) major nos modelos murinos de implante de parafina e esponja

Abstract

Because of the importance of monocytes-macrophages in Leishmania infection, we investigated in this work the main cytokines and chemokines involved in recruitment of these cells in animals susceptible and resistant to experimental infection with Leishmania major, in two models of chronic inflammation: (1) model implantation of paraffin (2) the sponge implant model. We investigated the histological profile, parasites and immune systems, through analysis of cytokines (IL-4, IL-10, TNF-, IFN- and IL-12) and chemokines (MCP-1/CCL2, MIP-1 / CCL3, RANTES/CCL5, CXCL10/IP10, CXCL9/MIG) involved in the recruitment of monocytes-macrophages and lymphocytes in the both immune response Th1 and Th2. Evaluations were performed at the time of 7 days after implantation of paraffin concomitant infection with L. Major and 7, 21 and 30 days after implantation of a sponge with concomitant infection by L. major. Was observed in both models studies, formation of granulation tissue consisting of large numbers of macrophages (epithelioid cells and giant cells), lymphocytes and newly formed blood vessels. C57BL/6 showed an increase of tissue parasitism in the animals Balb/c. The RT-PCR analysis of cytokines and chemokines confirmed the profile of resistance and susceptibility of C57BL/6 and Balb/c.