A novel wistar rat model of obesity-related nonalcoholic fatty liver disease induced by sucrose-rich diet
Carregando...
Data
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Minas Gerais
Descrição
Tipo
Artigo de periódico
Título alternativo
Primeiro orientador
Membros da banca
Resumo
The pathogenesis of nonalcoholic fatty liver disease (NAFLD) is not fully understood, and experimental models are an alternative
to study this issue. We investigated the effects of a simple carbohydrate-rich diet on the development of obesity-related NAFLD
and the impact of physical training on the metabolic abnormalities associated with this disorder. Sixty Wistar rats were randomly
separated into experimental and control groups, which were fed with sucrose-enriched (18% simple carbohydrates) and standard
diet, respectively. At the end of each experimental period (5, 10, 20, and 30 weeks), 6 animals from each group were sacrificed
for blood tests and liver histology and immunohistochemistry. From weeks 25 to 30, 6 animals from each group underwent
physical training. The experimental group animals developed obesity and NAFLD, characterized histopathologically by steatosis
and hepatocellular ballooning, clinically by increased thoracic circumference and body mass index associated with hyperleptinemia,
and metabolically by hyperglycemia, hyperinsulinemia, hypertriglyceridemia, increased levels of very low-density lipoprotein-
(VLDL-) cholesterol, depletion of the antioxidants liver enzymes superoxide dismutase and catalase, and increased hepatic levels
of malondialdehyde, an oxidative stress marker. Rats that underwent physical training showed increased high-density lipoprotein-
(HDL-) cholesterol levels. In conclusion, a sucrose-rich diet induced obesity, insulin resistance, oxidative stress, and NAFLD in
rats.
Abstract
Assunto
Ratos Wistar, Hepatopatia gordurosa não alcoólica, Sacarose na dieta, Obesidade
Palavras-chave
Wistar Rat, Nonalcoholic fatty liver disease, Sucrose-rich diet, Obesity
Citação
Curso
Endereço externo
https://www.hindawi.com/journals/jdr/2016/9127076/