Expressão e Localização da Folistatina e FLRG (gene relacionado a folistatina) na mama normal e patológica

Abstract

Follistatin and FLRG expression and localization in normal and pathological breast. Activins are growth factors from the transforming growth factor beta (TGFâ) superfamily that act through type I (ActRI) and type II (ActRII) receptors and have their effects antagonized by inhibins and follistatin. Activins have been related to mammary ductal elongation and lobular-alveolar differentiation in mice. Activins inhibits the proliferation of brest cancer cell lines in vitro. By the other size, folistatina and FLRG (folistatina related gene) are activins inhibitors. This paper aims to analyse Folistatin and FLRG expression and localization in human normal and pathological breast. There were used samples of breast tissue obtained from pacients submitted to mammoplasty, tumorectomy, quadrantectomy and mastectomy. The analysed samples were obtained from The Compared Pathology Laboratory files from ICB-UFMG. The samples were submitted to immunhistochemistry assay for folistatina and FLRG together with negative controls. The samples were analysed for colored intensity and colored area. The results were submitted to statistical descriptive analyses and were matched byKruskal-Wallis test. Values of P less than 0,05 were taken as significance and submitted to Dunn test of multiple matches. Folistatin and FLRG showed being present at normal and pathological human breasts. Folistatin expression seems being increased in fibroadenomas stromal cells (cytoplasm and nucleus) compared with ductal invasive carcinoma. FLRG expression seems being increased in ductal invasive carcinomaepithelial cells (cytoplasm and area) compared with normal controls.