Hydroxyapatite nanoparticles: preparation, characterization, and evaluation of their potential use in bone targeting: an animal study
Carregando...
Data
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Minas Gerais
Descrição
Tipo
Artigo de periódico
Título alternativo
Primeiro orientador
Membros da banca
Resumo
Objective
Hydroxyapatite is used as a drug-delivery system for bone therapy applications because of its biocompatibility, bioactivity, and osteoconductive properties. In addition, hydroxyapatite nanoparticles (HApN) might be used as a theranostic probe. The aim of this study was to prepare and characterize hydroxyapatite mesoporous nanoparticles, and radiolabel these nanoparticles with technetium-99m (99mTc). Moreover, biodistribution studies were carried out in healthy mice.
Materials and methods
HApN were synthesized and characterized. 99mTc-HApN was prepared by adding 99mTc-pertechnetate to a dispersion of HApN in the presence of stannous chloride. Radiochemical purity and in-vitro stability were determined. The circulation time of 99mTc-HApN was determined by measuring blood radioactivity in healthy mice. In addition, biodistribution studies were carried out in healthy mice at 1 and 4 h after injection.
Results
99mTc-HApN showed high radiochemical purity (98.7±0.2%) and in-vitro stability until 24 h. 99mTc-HApN levels in blood decreased in a biphasic manner, with an α half-life of 1.8 min and a β half-life of 126.9 min. High uptake was achieved in the liver and spleen because of the macrophage uptake. Furthermore, bone uptake was higher than that of the surrounding muscle, resulting in high bone-to-muscle ratios.
Conclusion
HApN were synthesized successfully with suitable characteristics for in-vivo applications. 99mTc-HApN was prepared and showed high stability. 99mTc-HApN presented increasing bone uptake over time, showing a higher affinity to bone tissues in contrast to surrounding muscle. The present results, together with further studies, may indicate a potential application of HApN as a nanocarrier for bone diseases.
Abstract
Assunto
Hidroxiapatita, Direcionamento ósseo, Animais, Nanopartículas radiomarcadas
Palavras-chave
Radiolabeled nanoparticles, Mesoporous hydroxyapatite nanoparticles, Controlled delivery system, Bone targeting
Citação
Departamento
Curso
Endereço externo
https://journals.lww.com/nuclearmedicinecomm/Abstract/2016/07000/Hydroxyapatite_nanoparticles__preparation,.12.aspx