Pulp response of rats submitted to bleaching and the use of different anti-inflammatory drugs
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Universidade Federal de Minas Gerais
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Artigo de periódico
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This study aimed to evaluate neuropeptide expression after bleaching treatment using histo pathological and immunohistochemical analyses and the effects of hydrocortisone and acet aminophen on pulp inflammation, sine dental bleaching and inflammation first occur, and
only then, the treatmentt. Sixty-three rats were divided into three groups (n = 21) according
to the pain-relieving therapy used: I-control; II-topical application of Otosporin for 10 min
after the bleaching treatment; III-oral administration of paracetamol 30 min before whitening
and then every 12h. In all the study groups, placebo gel was applied to the left upper jaw
(control) and a 35% H2O2-based whitening gel was applied to the right upper jaw for 45 min.
Seven animals from each group were euthanized at different time points: 0h after treatment,
24h, and 48h. After euthanasia, the first molar on each side was analyzed by histology and
immunohistochemistry to assess the degree of inflammation and verify the presence of the
neuropeptides, substance P (SP) and calcitonin gene-related peptide (CGRP). The data
were analyzed using the statistical nonparametric Kruskal-Wallis test followed by Dunn’s
test for individual comparisons. Extensive areas of necrosis were observed in the groups
that received bleaching treatment only, whereas reduced damage were obtained in the
group treated with Otosporin. The immunohistochemical analysis showed positive immuno labeling in all groups, including the control, but this was stronger in the groups that received
bleaching treatment. The best results were obtained in the group that received treatment
with Otosporin. The use of Otosporin after dental bleaching minimized the side effects of
this treatment.
Abstract
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Tooth bleaching, Immunohistochemistry, Hidrocortisona, Acetaminofén, Inflammation
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https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0210338