Identificação de resíduos de isoniazida no leite bovino pela cromatografia liqüída de alta eficiência

Abstract

High Performance Liquid Chromatography (HPLC) was used in the identification of lsoniazid (Hydrazid Isonicotinic Acid) in milk of cattle treated with 25 mg/kg/day every other day. The effect of pasteurization was also verified on the residual concentration of the drug in the milk of these animals. Excretion of the drug on the Milk showed a cyclical variation, with an average increase of 1104,48 ug/l one day after application ofthe drug, and an average decrease after two days of` application for 104,12 ug/l. The technique used did not identify any residue of` isoniazid in the milk four days after the last application of` the drug. It was observed that the weight and the milk production of` the animals studied influenced the amount of drug excreted. Pasteurization decreased, the residue of` isoniazid in the milk by an average of 47,07%.