Mycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients
Carregando...
Data
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Minas Gerais
Descrição
Tipo
Artigo de periódico
Título alternativo
Primeiro orientador
Membros da banca
Resumo
Previously we showed that 65-kDa Mycobacterium leprae heat shock protein (Hsp65) is a target for the development of a tuberculosis vaccine. Here we evaluated peripheral blood mononuclear cells (PBMC) from healthy individuals or tuberculosis patients stimulated with two forms of Hsp65 antigen, recombinant DNA that encodes Hsp65 (DNA-HSP65) or recombinant Hsp65 protein (rHsp65) in attempting to mimic a prophylactic or therapeutic study in vitro, respectively. Proliferation and cytokine-producing CD4C or CD8C cell were assessed by flow cytometry. The CD4C cell proliferation from healthy individuals was stimulated by DNA-HSP65 and rHsp65, while CD8C cell proliferation from healthy individuals or tuberculosis patients was stimulated by rHSP65. DNA-HSP65 did not improve the frequency of IFN gammaC cells from healthy individuals or tuberculosis patients. Furthermore, we found an increase in the
frequency of IL-10-producing cells in both groups. These findings show that Hsp65 antigen activates human lymphocytes and plays an immune regulatory role that should be addressed as an additional antigen for the development of antigen-combined therapies.
Abstract
Assunto
Interferon beta, Interleucina-10, Tuberculose, Vaccinia
Palavras-chave
Interferon beta, Interleucina-10, Tuberculosis, Vacine
Citação
Curso
Endereço externo
https://www.tandfonline.com/doi/full/10.1080/21645515.2016.1264547