Mycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients

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dc.creatorPryscilla Fanini Wowk
dc.creatorFlávia Dias Coelho Silva
dc.creatorSolange Alves Vinhas
dc.creatorOlindo Assis Martins Filho
dc.creatorMoisés Palaci
dc.creatorCélio Lopes Silva
dc.creatorVânia Luiza Deperon Bonato
dc.creatorLuís Henrique Franco
dc.creatorDenise Morais da Fonseca
dc.creatorMarina Oliveira Paula
dc.creatorÉlcio Dos Santos Oliveira Vianna
dc.creatorAna Paula Barbosa Wedling
dc.creatorValéria Maria Augusto
dc.creatorSilvana Maria Elói Santos
dc.creatorAndréa Teixeira Carvalho
dc.date.accessioned2023-06-15T19:22:47Z
dc.date.accessioned2025-09-08T23:18:50Z
dc.date.available2023-06-15T19:22:47Z
dc.date.issued2017-02-21
dc.format.mimetypepdf
dc.identifier.doi10.1080/21645515.2016.1264547
dc.identifier.issn21645515
dc.identifier.urihttps://hdl.handle.net/1843/54960
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofHuman Vaccines & Immunotherapeutics
dc.rightsAcesso Aberto
dc.subjectInterferon beta
dc.subjectInterleucina-10
dc.subjectTuberculose
dc.subjectVaccinia
dc.subject.otherInterferon beta
dc.subject.otherInterleucina-10
dc.subject.otherTuberculosis
dc.subject.otherVacine
dc.titleMycobacterial hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients
dc.typeArtigo de periódico
local.citation.epage1050
local.citation.issue5
local.citation.spage1040
local.citation.volume13
local.description.resumoPreviously we showed that 65-kDa Mycobacterium leprae heat shock protein (Hsp65) is a target for the development of a tuberculosis vaccine. Here we evaluated peripheral blood mononuclear cells (PBMC) from healthy individuals or tuberculosis patients stimulated with two forms of Hsp65 antigen, recombinant DNA that encodes Hsp65 (DNA-HSP65) or recombinant Hsp65 protein (rHsp65) in attempting to mimic a prophylactic or therapeutic study in vitro, respectively. Proliferation and cytokine-producing CD4C or CD8C cell were assessed by flow cytometry. The CD4C cell proliferation from healthy individuals was stimulated by DNA-HSP65 and rHsp65, while CD8C cell proliferation from healthy individuals or tuberculosis patients was stimulated by rHSP65. DNA-HSP65 did not improve the frequency of IFN gammaC cells from healthy individuals or tuberculosis patients. Furthermore, we found an increase in the frequency of IL-10-producing cells in both groups. These findings show that Hsp65 antigen activates human lymphocytes and plays an immune regulatory role that should be addressed as an additional antigen for the development of antigen-combined therapies.
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICA
local.publisher.initialsUFMG
local.url.externahttps://www.tandfonline.com/doi/full/10.1080/21645515.2016.1264547

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