The participation of tumor residing pericytes in oral squamous cell carcinoma
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Membros da banca
Resumo
Pericytes are perivascular cells related to vessel structure and angiogenesis that can interact with neoplastic cells, interfering with cancer progression and outcomes. This study focused on the characterization of pericytes in oral squamous cell carcinoma (OSCC) using clinical samples and a transgenic mouse model of oral carcinogenesis. Nestin-/NG2+ (type-1) and nestin+/NG2+ (type-2) pericytes were analyzed by direct fluorescence after induction of oral carcinogenesis (4-nitroquinoline-1-oxide). Gene expression of neuron glial antigen-2 (NG2), platelet-derived growth factor receptor beta (PDGFR-β), and cluster of differentiation 31 (CD31) was examined in human OSCC tissues. The protein expression of von Willebrand factor and NG2 was assessed in oral leukoplakia (i.e., oral potentially malignant disorders) and OSCC samples. Additionally, clinicopathological aspects and survival data were correlated and validated by bioinformatics using The Cancer Genome Atlas (TCGA). Induction of carcinogenesis in mice produced an increase in both NG2+ pericyte subsets. In human OSCC, advanced-stage tumors showed a significant reduction in CD31 mRNA and von Willebrand factor-positive vessels. Low PDGFR-β expression was related to a shorter disease-free survival time, while NG2 mRNA overexpression was associated with a reduction in overall survival, consistent with the TCGA data. Herein, oral carcinogenesis resulted in an increase in NG2+ pericytes, which negatively affected survival outcomes.
Abstract
Assunto
Neoplasms, Pericytes, Mouth neoplasms, Carcinogenesis, Gene expression, Proteins, Survival, RNA, messenger, Platelet endothelial cell adhesion molecule-1, Squamous cell carcinoma of head and neck, Leukoplakia, oral, Evaluation study, Receptor, platelet-derived growth factor beta, Nestin, Von Willebrand factor
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Oral cancer, Cancer
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Endereço externo
https://www.nature.com/articles/s41598-023-32528-1