1. Repositório Institucional da UFMG
2. Publicações Científicas e Culturais
3. Artigo de Periódico

Type:	Artigo de Periódico
Title:	Radiation therapy reduced blood levels of LDH, HIF-1α, and miR-210 in OSCC
Authors:	Marcela Gonçalves de Souza Sabrina Ferreira de Jesus Eloá Mangabeira Santos Emisael Stênio Batista Gomes Arlen de Paulo Santiago Filho Eliane Macedo Sobrinho Santos Luiz Henrique da Silveira Sergio Henrique Sousa Santos Alfredo Maurício Batista de Paula Lucyana Conceição Farias André Luiz Sena Guimarães
Abstract:	Radiation Therapy (RT) is a treatment option for a large number of neoplasias. However, the effect of RT on the level of hypoxia markers is poorly understood. The present study aimed to investigate the effect of RT on the levels of hypoxic markers in Oral squamous cell carcinoma (OSCC). Evaluation of HIF-1α and miR-210 levels in OSCC was performed. Then a proteomic analysis was performed to identify candidate hypoxic targets of RT. To validate proteomic studies, the effect of RT on HIF-1α, miR-210, PDH-A and LDH-A levels under hypoxia was assessed by qRT-PCR. The impact of RT in hypoxia markers was evaluated in patients to confirm in vitro results. An increase in the HIF-1α levels was observed in OSCC. RT reduced OSCC cell proliferation and migration. Interestingly, hypoxia could revert the effect of radiation on OSCC phenotype. However, proteomics analyses suggested that LDH is one of the critical targets of RT even in hypoxia. Moreover, RT decreased HIF-1α, miR-210, and LDH even in hypoxia. The current study demonstrated that hypoxia could revert the effects of RT in the OSCC context. However, RT reduces the levels HIF-1α, miR-210 and LDH in vivo and in vitro. The consequences of RT in blood should be carefully investigated.
Subject:	Câncer - Radioterapia Lactato desidrogenase Carcinoma de células escamosas
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	10.1007/s12253-018-0517-2
URI:	http://hdl.handle.net/1843/44433
Issue Date:	8-Nov-2018
metadata.dc.url.externa:	https://link.springer.com/article/10.1007/s12253-018-0517-2
metadata.dc.relation.ispartof:	Pathology and Oncology Research
Appears in Collections:	Artigo de Periódico

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.