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http://hdl.handle.net/1843/45466| Type: | Artigo de Periódico |
| Title: | Evaluation of the cytotoxicity and biocompatibility of new resin epoxy-based endodontic sealer containing calcium hydroxide |
| Authors: | Luciano Tavares Angelo Cintra Francine Benetti Índia Olinta de Azevedo Queiroz Luciana Louzada Ferreira Loiane Massunari Carlos Roberto Emerenciano Bueno Sandra Helena Penha de Oliveira Gomes-Filho |
| Abstract: | Introduction: Many endodontic sealers are available, but the search for the ideal sealer continues. This study evaluated the cytotoxicity and biocompatibility of Sealer Plus, a new resin epoxy–based endodontic sealer con- taining calcium hydroxide. AH Plus, Endofill, and Simpli- Seal endodontics sealers were used for comparison. Methods: L929 fibroblasts were cultured, and an MTT assay was used to determine the cytotoxicity of the sealer extracts at 6, 24, 48, and 72 hours. Tubes contain- ing materials or empty tubes for control were inserted into the subcutaneous tissues of 20 rats. After 7 and 30 days, the rats were killed, and the tubes were removed with the surrounding tissues for histologic analysis. The data were submitted to statistical tests (P < .05). Results: Undiluted Sealer Plus exhibited less cytotoxicity compared with other undiluted extracts at 6 hours (P < .05), and cell viability was higher for all Sealer Plus extracts after 24 hours (P < .05). At 48 hours, the undiluted and ½ Sealer Plus dilution were the ex- tracts with less cytotoxicity (P < .05). At 72 hours, cell viability was higher for the undiluted and ½ Sealer Plus dilution compared with the other sealers (P < .05). At 7 days, Endofill and SimpliSeal had higher inflammation compared with the control and Sealer Plus (P < .05); AH Plus had moderate inflammation (P > .05). At 30 days, control, Sealer Plus, and AH Plus had less inflammation (P < .05). The fibrous capsule was thick at 7 days and thin at 30 days, except for SimpliSeal. Conclusions: In general, Sealer Plus promoted greater cell viability and was more biocompatible compared with the other sealers. |
| Subject: | Materials testing Calcium hydroxide Epoxy resins Noxae |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | 10.1016/j.joen.2017.07.016 |
| URI: | http://hdl.handle.net/1843/45466 |
| Issue Date: | Dec-2017 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0099239917309056?via%3Dihub |
| metadata.dc.relation.ispartof: | Journal of Endodontics |
| Appears in Collections: | Artigo de Periódico |
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