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Type:	Artigo de Periódico
Title:	Saliva biomonitoring using LPME-GC/MS method to assess dentistry exposure to plasticizers
Authors:	José Messias Gomes Tatiana Fernandes Araujo Almeida Tarcília Aparecida da Silva Zenilda de Lourdes Cardeal Helvécio Costa Menezes
Abstract:	Bisphenol A and phthalates are endocrine disruptors widely used as chemical additives mainly in plastic products, including materials for dentistry procedures. Besides, many plasticizers have been associated with important diseases requiring performed methods for their quantification. In the present study, an alternative method for the determination of bisphenol A (BPA) and phthalate metabolites in saliva was developed and validated using hollow fiber liquid phase microextraction (HF-LPME) for sample preparation and gas chromatography coupled to ion trap mass spectrometry (GC/MS) for analysis. A mixture of octanol and ethyl octanoate (1:1 v/v) was used as an acceptor phase in hollow fiber to extract the analytes from saliva samples. A Doehlert design was performed to optimize the variable sample agitation and extraction time. The HF-LPME-GC/MS method developed for saliva analysis showed good selectivity, linearity (R2 > 0.900), and precision (CV = 0.86–18.68%). Limits of detection and quantification ranged from 0.03 to 0.53 μg L−¹ and 0.09 to 1.78 μg L−¹, respectively. A high concentration of BPA in the oral cavity and oropharyngeal space is a warning of the possible association with the main cancer of the mouth. The method developed and validated was applied to patients with oral squamous cell carcinoma (study group, n = 16) and patients who did not present any oral lesion (control group, n = 16). A principal component analysis was performed and showed a tendency for the association between oral squamous cell carcinoma (OSCC) and plasticizers.
Subject:	Química analítica Extração (Química) Cromatografia de gás Espectrometria de massa Saliva Preparação de amostra (Química) Tratamento dentário
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ICX - DEPARTAMENTO DE QUÍMICA FAO - FACULDADE DE ODONTOLOGIA
Rights:	Acesso Restrito
metadata.dc.identifier.doi:	https://doi.org/10.1007/s00216-020-02908-x
URI:	http://hdl.handle.net/1843/48867
Issue Date:	2020
metadata.dc.url.externa:	https://link.springer.com/article/10.1007/s00216-020-02908-x
metadata.dc.relation.ispartof:	Analytical and Bioanalytical Chemistry
Appears in Collections:	Artigo de Periódico

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