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http://hdl.handle.net/1843/54409| Type: | Artigo de Periódico |
| Title: | Caga-positive helicobacter pylori strain containing three epiya c phosphorylation sites produces increase of g cell and decrease of d cell in experimentally infected gerbils (meriones unguiculatus) |
| Authors: | Moacir Ferreira Júnior Sérgio de Assis Batista Rafael Calvão Barbuto Adriana Dias Gomes Dulciene Maria Magalhães Queiroz Ivana Duval Araújo Marcelo Vidigal Caliari |
| Abstract: | Purpose: Human infection by Helicobacter pylori is associated with an increase in the number of gastrin producing G cells and a concomitant decrease of somatostatin-producing D cells. However, to our knowledge, changes in G and D cell numbers in response to infection with H. pylori CagA-positive strains containing different number of EPIYA-C phosphorylation sites have not been analyzed to date. Therefore,the aim of this study was to perform a quantitative analysis of the number of G and D cells in Mongolian gerbils challenged with H. pylori strains with different numbers of EPIYA-C motifs.Materials and methods: Mongolian gerbils were inoculated with isogenic H. pylori strains containing one to three phosphorylation sites. Mucosal fragments were evaluated by morphometry and immunohistochemistry using primary polyclonal rabbit anti-gastrin and anti-somatostatin antibodies. Positive cells were counted using an image analyzer.Results: Forty-five days after infection, there was a decrease in the number of D cells and an increase inthe G/D cell ratio in the group with three EPIYA-C. Six months after infection, there was a progressive and significant increase in the number of G cells and in the G/D cell ratio, with a concomitant decrease in thenumber of D cells, especially in the three EPIYA-C group.Conclusions: CagA-positive H. pylori strains containing a large number of EPIYA-C phosphorylation sites induce a decrease in D cell number and an increase in G cell number and G/D ratio, which were correlated with the number of inflammatory cells of the lamina propria. |
| Subject: | Helicobacter pylori Células Secretoras de Gastrina Células Secretoras de Somatostatina |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICB - DEPARTAMENTO DE PATOLOGIA MED - DEPARTAMENTO DE CIRURGIA MED - DEPARTAMENTO DE PROPEDÊUTICA COMPLEMENTAR |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | 10.1016/j.advms.2016.02.003 |
| URI: | http://hdl.handle.net/1843/54409 |
| Issue Date: | 23-Feb-2016 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S1896112616000158?via%3Dihub |
| metadata.dc.relation.ispartof: | Advances in Medical Sciences |
| Appears in Collections: | Artigo de Periódico |
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