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3. Artigo de Periódico

Type:	Artigo de Periódico
Title:	Development of a graphene-based biosensor for detecting recombinant cyanovirin-N
Authors:	Pedro Rodrigues de Almeida III André Melro Murad Luciano Paulino da Silva Elibio Leopoldo Rech Filho Elmo Salomão Alves
Abstract:	We present a graphene-based biosensor selective to recombinant cyanovirin-N (rCV-N),an antiviral protein that has proven to be an effective microbicide to inhibit HIV replication. We modified the graphene monolayer devices with 1-pyrenebutanoic acid succinimidyl ester, which interacts with both graphene and the primary and secondary amines of antibodies. By monitoring the change in the electrical resistance of the device, we were able to detect rCV-N in solutions in the range of 0.01 to 10 ng/mL, and found that the detection limit was 0.45 pg/mL, which is much smaller than that obtained with currently available techniques. This is important for applications of this microbicide against HIV, since it may be produced at a large scale from soya bean seeds processed using the available industrial processing technologies. The sensor showed high sensitivity, selectivity, and reproducibility.
Subject:	Grafeno Sensores
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ICX - DEPARTAMENTO DE FÍSICA
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.3390/bios10120206
URI:	http://hdl.handle.net/1843/58304
Issue Date:	2020
metadata.dc.url.externa:	https://www.mdpi.com/2079-6374/10/12/206
metadata.dc.relation.ispartof:	Biosensors
Appears in Collections:	Artigo de Periódico

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