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http://hdl.handle.net/1843/80541| Type: | Artigo de Periódico |
| Title: | Immunomodulatory and neuroprotective effect of cryopreserved allogeneic mesenchymal stem cells on spinal cord injury in rats |
| Authors: | Isabel Rodrigues Rosado Alfredo de Miranda Goes Eliane Goncalves de Melo Pablo Carvalho E. G. L. Alves T. M. Tagushi J. L. Carvalho Juneo de Freitas Silva M. S. L. Lavor K. M. Oliveira Rogéria Serakides |
| Abstract: | This study aimed to evaluate the immunomodulatory and neuroprotective effects of allogeneic and cryopreserved mesenchymal stem cells (MSCs) on spinal cord injury. A total of 120 rats were distributed into the following groups: negative control (NC) - without injury, positive control (PC) - with injury without treatment, and group treated with MSC (GMSC) - with injury and treated. Motor function was evaluated by the BBB test at 24, 48, and 72 h and at 8 and 21 postoperative days. Spinal cords were evaluated by histopathology and immunohistochemistry to determine the expression of CD68, NeuN, and GFAP. IL-10, TNF-α, IL-1β, TGF-β, BDNF, GDNF, and VEGF expression was quantified by RT-PCR. The GMSC presented higher scores for motor function at 72 h and 8 and 21 days after injury, lower expression of CD68 at 8 days, and lower expression of GFAP at 21 days compared to the PC. In addition, higher expression of NeuN and lower degeneration of the white matter occurred at 21 days. The GMSC also showed higher expression of IL-10 24 h after injury, GDNF at 48 h and 8 days, and VEGF at 21 days. Moreover, lower expression of TNF-α was observed at 8 and 21 days and TGF-β at 24 h and 21 days. There were no differences in the expression of IL-1β and BDNF between the GMSC and PC. Thus, cryopreserved MSCs promote immunomodulatory and neuroprotective effects in rats with spinal cord injury by increasing IL-10, GDNF, and VEGF expression and reducing TNF-α and TGF-β expression. |
| Subject: | Neurologia Inflamação Medicina veterinária Medicina Terapia celular |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA VETER - ESCOLA DE VETERINARIA |
| Rights: | Acesso Aberto |
| URI: | http://hdl.handle.net/1843/80541 |
| Issue Date: | 22-Mar-2017 |
| metadata.dc.url.externa: | https://www.funpecrp.com.br/gmr/year2017/vol16-1/pdf/gmr-16-01-gmr.16019555.pdf |
| metadata.dc.relation.ispartof: | Genetics and Molecular Research |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Immunomodulatory and neuroprotective effect.pdf | 6.67 MB | Adobe PDF | View/Open |
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