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http://hdl.handle.net/1843/81836| Type: | Artigo de Periódico |
| Title: | Effect of porous tantalum on the biological response of human peripheral mononuclear cells exposed to Porphyromonas gingivalis |
| Authors: | Eduardo Frois Temponi Paulo Eduardo Alencar Souza Giovanna Ribeiro Souto Luísa Mourão Dias Magalhães Walderez Ornelas Dutra Kenneth John Gollob Tarcília Aparecida da Silva Rodrigo Villamarim Soares |
| Abstract: | Aim: To evaluate biological behavior of human peripheral mononuclear cells (PBMC) in contact with porous tantalum (PT) and Porphyromonas gingivalis (Pg). Methods: Pg was incubated for 8 hours. The groups formed were: PBMC (control), PBMC + PT, PBMC + Pg and PBMC + PT + Pg. Cell viability was evaluated using MTT assay. The morphology and adhesion of PBMC to PT was evaluated using scanning electron microscopy. Expression of interleukin (IL)-10, transforming growth factor (TGF)-β, matrix metallopeptidase (MMP)-9 and receptor activator of nuclear factor-κΒ ligand (RANKL) was determined by enzyme-linked immunosorbent assay. Results: MTT assay revealed that PT did not interfere in the mitochondrial activity of PBMC (P > .05). Scanning electron microscopy showed the adherence of PBMC to PT. IL-10 levels in PBMC + PT were similar to PBMC and lower than PBMC + Pg. TGF-β levels in PBMC + PT were higher than PBMC and PBMC + Pg. MMP-9 levels in PBMC + PT were similar to PBMC and lower than PBMC + Pg and PBMC + PT + Pg. RANKL levels in PBMC + PT were lower than in PBMC. Conclusion: PT did not affect PBMC viability, allowed cell adhesion, reduced expression of RANKL and enhanced TGF-β in comparison with the control group. |
| Subject: | Cytokines Porphyromonas gingivalis Leukocytes, mononuclear Behavior Biology Cell adhesion Transforming growth factor beta RANK ligand Tantalum |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE CLÍNICA ICB - DEPARTAMENTO DE MORFOLOGIA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1111/jicd.12472 |
| URI: | http://hdl.handle.net/1843/81836 |
| Issue Date: | Nov-2019 |
| metadata.dc.url.externa: | https://onlinelibrary.wiley.com/doi/10.1111/jicd.12472 |
| metadata.dc.relation.ispartof: | Journal of investigative and clinical dentistry |
| Appears in Collections: | Artigo de Periódico |
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