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Type:	Artigo de Periódico
Title:	Bone strength is reduced in a neonatal androgenized rat model
Authors:	Lady Katerine Serrano Mujica Antônio Carlos Shimano Alfredo Quites Antoniazzi Melissa Orlandin Premaor Fabio Vasconcellos Comim Carolina dos Santos Amaral Fernanda Soldatelli Valente Ligia Gomes Miyazato Soraia Macari Tarcília Aparecida da Silva Breno Rocha Barrioni Bruna Leonel Carlos Guilherme Jafroni Alves Silva
Abstract:	Background: Whether polycystic ovary syndrome (PCOS) affects bone health during a woman's lifespan remains controversial. An androgenized rodent model replicated many metabolic and reproductive features of women with PCOS, and we aimed to use it to investigate the impact of androgens on microarchitecture (by micro-CT), bone mechanical strength, bone formation and resorption markers in rats with intact ovaries (SHAM) who underwent oophorectomy. Methods: Wistar rats (Rattus norvegicus albinus) were employed for the experiments in this study. The protocol of androgenization consisted of the application of 1.25 mg s.c. testosterone propionate beteween days 2–5 of life, while the controls received the same amount of corn oil s.c. as previously established. Androgenized SHAM rats exhibited chronic anovulation identified by vaginal cytology and a reduction in the proportion of corpus luteum in the ovary in comparison to control SHAM rats. The realization of the ovariectomy or SHAM procedure occurred on Day 100 of life. All groups (n = 8) were followed-up for 180 days to address the study endpoints. Results: Micro-CT from androgenized female rats (SHAM) showed a divergence between the trabecular and cortical bone profiles. Compared to SHAM controls, these rats had an increase in trabecular bone mass with a diminution in bone resorption C-terminal telopeptide of type 1 collagen (CTX) (p < 0.05), a concomitant decrease in cortical area and thickness in the femur, and a reduction in the strength of the femur on the mechanical test (p < 0.01). Conclusions: Our results suggest that a reduction in the cortical thickness and cortical area observed in PCOS model rats was associated with a reduced strength of the femur, despite increased trabecular formation. Ovariectomy in the androgenized OVX group limited the progression rate of cortical bone loss, resulting in bone resistance and cortical thickness comparable to those observed in the control OVX group.
Subject:	Bone and bones Polycystic ovary syndrome Mechanical tests Bone resorption Femur Collagen type I Ovariectomy
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	FAO - DEPARTAMENTO DE CLÍNICA FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA MED - DEPARTAMENTO DE CLÍNICA MÉDICA
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.1016/j.bonr.2023.101710
URI:	http://hdl.handle.net/1843/82349
Issue Date:	Dec-2023
metadata.dc.url.externa:	https://www.sciencedirect.com/science/article/pii/S235218722300058X#ks0005
metadata.dc.relation.ispartof:	Bone reports
Appears in Collections:	Artigo de Periódico

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